Tim Blake Nelson as Stodgy, another 1 of Experion's pure evil grasshopper henchman (voice).Michael Keaton as Jumper, 1 of Experion's pure evil grasshopper henchman (voice).Matthew Lillard as James, Flik's brother and Atta and Dot's brother in law (voice).Alec Baldwin as Henry, Flik and James's father and Atta and Dot's father in law (voice).Neil Ross as Experion, the main antagonist (voice).Mindy Kaling as Sophie, a female ladybug and Francis's 1 true love (voice).John Cleese as Manny (voice, replacing the late Jonathan Harris respectively).Steven Kynman as Cornelius (voice, replacing David Ossman respectively).Jim Cummings as Thorny (voice, replacing the late Alex Rocco respectively).Soil (voice, replacing the late Roddy McDowell respectively) Michael McShane as Tuck and Roll (voices).Drew Barrymore as Gypsy (voice, replacing the late Madeline Kahn respectively).Richard Kind as Molt, who's friends with the ants and is a good guy ever since Hopper's death (voice).
Jerome Ranft as Heimlich (voice, replacing his late brother, the great and talented, Joe Ranft respectively).Hayden Panettiere as Dot, who's now 17 years old (voice).Flik and the entire insect gang prepare themselves to fight against Experion, the dark and pure evil grasshopper leader and the cousin of Hopper who was killed off and eaten by the bird's babies in the 1998 film.